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. 2018 Feb 13;49:15. doi: 10.1186/s13567-018-0508-z

Figure 5.

Figure 5

Effects of selenium supplementation on OTA-induced autophagy in PCV2-infected PK-15 cells. A PK-15 cells were cultured with or without selenium for 12 h then incubated with PCV2 and 0.1 μM OTA for another 60 h. After harvest, the expressions of LC3, ATG5, p-AKT, AKT, p-mTOR, mTOR and β-actin were analyzed by Western blotting as described in Materials and methods. Data are presented as mean ± SE of three independent experiments. Significance compared with control, *P < 0.05 and **P < 0.01. Significance compared with OTA, #P < 0.05 and ##P < 0.01. B PK-15 cells were transfected with GFP-LC3 plasmid. Then PK-15 cells were cultured with or without selenium for 12 h and incubated with PCV2 and 0.1 μM OTA for another 60 h and the fluorescence signals were visualized by confocal immunofluorescence microscopy (Scale bar: 10 μm). The average number of LC3 puncta in each cell was determined from at least 100 cells in each group. Data are presented as mean ± SE of three independent experiments. Significance compared with control, *P < 0.05 and **P < 0.01. Significance compared with PCV2, #P < 0.05 and ##P < 0.01.