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. 2018 Feb 13;49:16. doi: 10.1186/s13567-018-0513-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Assaying the poly (I:C) and CVP1 complexes by gel retardation electrophoresis and detection the relative mRNA levels of IFN-β by real-time PCR. A Testing the complex of poly (I:C) and CVP1 was conducted by electrophoresis and ethidium bromide staining. Lane 1: production of CVP1 peptide co-incubated with poly (I:C); Lane 2: poly (I:C) only; B The mRNA level of IFN-β obtained from the complexes of CVP1 peptide and TAT conjugated with poly (I:C), respectively, TAT and CVP1 peptide only, transfection reagent with poly (I:C) and blank cells. Single asterisk (*) and double asterisks (**) indicated P < 0.05 and P < 0.01, respectively.