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. 2018 Feb 13;49:16. doi: 10.1186/s13567-018-0513-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of temperature and endocytosis inhibitor on CVP1 internalization. Confocal images of HCT116 cells incubated with CVP1 (10 µM) at 4 °C (A) and 37 °C (B); C the flow cytometry data for the effect of temperature were indicated as mean ± SD. Single asterisk (*) and double asterisks (**) indicated P < 0.05 and P < 0.01, respectively. D The effect of inhibitors on endocytosis is shown as mean ± SD.