Saccharification performance of CbGH5 and the mixed commercial cellulase+xylanase of Novozymes. Contents of lignin, cellulose, hemicellulose, glucose and xylose were measured before and after enzymatic hydrolysis, on the straws of wheat (A), rice (B), corn (C) and oilseed rape (D), as well as spent mushroom substrates made from the straws of wheat (E), rice (F), corn (G) and oilseed rape (H). White bar: raw material before hydrolysis. Light grey bar: hydrolysed by the mixed Novozymes cellulase+xylanase. Dark grey bar: hydrolysed by CbGH5. The folds of glucose and xylose released by CbGH5 compared with the mixed Novozymes cellulase+xylanase are labelled in the figures. The hydrolysis reaction was carried out for 2 h. The pH and temperature conditions were 4.8, 45 °C for the mixed Novozymes cellulase plus xylanase, and 8.5, 90 °C for CbGH5 respectively. The values of the data bars in the figures are provided in the supplementary data (Table S6).