Table 1.
Summary of the bifunctional cellulase–xylanase enzymes characterized to date
| Protein name/ID | Family | Source | GenBank accession number | References | Maximum activity level and catalytic efficiency (at optimum pH and temperature) | |
|---|---|---|---|---|---|---|
| EG‐CMC | XYN‐bw | |||||
| Cel5 | GH5_2 | Hahella chejuensis KCTC 2396 | ABC30636 | (Ghatge et al., 2014) | 77.19 U mg−1, 1.63 × 10−2 ml mg−1 s−1 a (at pH 6.5, 65 °C) | 9.19 U mg−1, 1.83 × 10−3 ml mg−1 s−1 (at pH 5.5, 55 °C) |
| rBhcell‐xyl | GH5_4 | Bacillus halodurans C‐125 | BAB04322 | (Rattu et al., 2016) | 16.88 U mg−1, 2.85 ml mg−1 s−1 a (at pH 6, 60 °C) | 6.7 U mg−1, 10.95 ml mg−1 s−1 a (at pH 6, 60 °C) |
| Cel5A | GH5_4 | Bovine rumen metagenome | AFO64636 | (Rashamuse et al., 2013) | 2.73 U ml−1, N/A (at pH 9, 65 °C) | N/A |
| Cel5B | GH5_4 | Bovine rumen metagenome | AFO64637 | (Rashamuse et al., 2013) | 2.1 U ml−1, N/A (at pH 9, 65 °C) | N/A |
| 558318_19410/MH‐20 | GH5_4 | Cow rumen metagenome | ADX05703 | (Hess et al., 2011) | N/A | N/A |
| Cel28a | GH5_4 | Goat rumen metagenome | AHW46443 | (Cheng et al., 2015) | 20.56 U mg−1 (at pH 5, 50 °C) | 9.02 U mg−1 (at pH 5, 50 °C) |
| RuCelA | GH5_4 | Yak rumen metagenome | ADK55024 | (Chang et al., 2011) | 54.3 U mg−1, N/A (at pH 5, 50 °C) | 264.1 U mg−1, N/A (at pH 7, 65 °C) |
| CtCel5E | GH5_25 | Ruminiclostridium thermocellum ATCC 27405 | ABN52701 | (Yuan et al., 2015) | 736.2 U mg−1 g, 12.4 ml mg−1 s−1 a (at pH 5, 50 °C) | 255.8 U mg−1, 3.2 ml mg−1 s−1 a (at pH 6, 60 °C) |
| CbGH5 | GH5_46 | Chryseobacterium sp. HT1 | ANQ80467 | This study | 3237 U mg−1, 1.11 × 105 ml mg−1 s−1 (at pH 9, 90 °C) | 1793 U mg−1, 1.17 × 104 ml mg−1 s−1 (at pH 9, 90 °C) |
| MtEG7a | GH7 | Myceliophthora thermophile ATCC 42464 | XM_003663393 | (Karnaouri et al., 2014) | 177 U mg−1, 0.314 ml mg−1 s−1 a (at pH 5, 60 °C) | 4 U mg−1, N/A (at pH 5, 60 °C) |
| Cel7B | GH7 | Trichoderma reesei QM9414 | AAA34212 | (Nakazawa et al., 2008) | 65 U mg−1, N/A (at pH 3, 50 °C) | 3.9 U mg−1 a, N/A (at pH 3, 50 °C) |
| ThCel7B | GH7 | Trichoderma harzianum IOC‐3844 | N/A | (Pellegrini et al., 2015) | 26 U mg−1, N/A (at pH 3, 55 °C) | 12 U mg−1, N/A (at pH 3, 55 °C) |
| EgXA | GH10 | Ampullaria crossean | AAP31839 | (Ding et al., 2008) | 7.5 U mg−1, N/A (at pH 5, 40 °C) | 391.8 U mg−1, N/A (at pH 6, 45 °C) |
| 3811766_163670/TW‐8 | GH10 | Cow rumen metagenome | ADX05679 | (Hess et al., 2011) | N/A | N/A |
| 3663344_18020/TW‐23 | GH10 | Cow rumen metagenome | ADX05674 | (Hess et al., 2011) | N/A | N/A |
| 3789863_192950/TW‐24 | GH10 | Cow rumen metagenome | ADX05745 | (Hess et al., 2011) | N/A | N/A |
| AgEG | GH61 | Armillaria gemina KJS114 | N/A | (Jagtap et al., 2014) | 1160 U mg−1, 3590 ml mg−1 s−1 (at pH 5, 60 °C) | 1392 U mg−1 a, N/A (at pH 5, 60 °C) |
N/A not available since the authors did not measure the parameters.
a. Value is calculated or deduced from original data given in the article.