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Rac1 shRNA decreases the level of Rac1. (A) mRNA level of Rac1 in each group was measured using quantitative real-time PCR. The relative mRNA level of Rac1 was calculated using 2−ΔΔCt method. (B) The protein level of Rac1 was detected by Western blot with GAPDH as the internal reference. All experiments were repeated 3 times. The results are presented as mean ±SD. *** P<0.001 compared with the negative control group.
