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. 2018 Feb 7;24:768–781. doi: 10.12659/MSM.907468

Figure 3.

Figure 3

Rac1 shRNA inhibits the proliferation of HSCC cells and arrests their cell cycle. (A) The cell viability of FaDu cells was detected using MTT assay. (B) The cell cycle distribution of FaDu cells was detected by flow cytometry. (C) The protein level of cyclinB in each group was detected by Western blot analysis. GAPDH served as the internal reference. (D) The protein level of cyclinD1 in each group was detected by Western blot analysis using GAPDH as the internal reference. (E) Western blot analysis was used to detect the protein level of cyclinE in each group. Each experiment was repeated 3 times. The results are presented as mean ±SD. * P<0.05, ** p<0.01, *** p<0.001 compared with the negative control group.