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. 2018 Feb 9;11:34. doi: 10.3389/fnmol.2018.00034

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Copyright © 2018 Wang, Hou, Song, Zhang, Zhang, Cao, Chen, Sang, Lin and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lpar2b regulates Yap1 activity. (A,A′) Whole-mount ISH detects the mRNA of yap1 in embryos at 34 hpf. (A′) High-magnification of the pLLP in (A). (B,C) Epifluorescence images of control- or yap1 MO-injected Et(gata2:EGFP)^mp189b embryos at 48 hpf. (D) Quantification of the NM number at 48 hpf and number of pLLP cells at 32 hpf in control- or yap1 MO-injected embryos. (E–F′) Confocal images of the pLLP stained with DAPI (blue) and p-Yap1 (green) at 32 hpf. (G,H) ISH shows a decreased prox1a expression in the pLLP of Lpar2b morphants. The pLLP is outlined by dots. ^**p < 0.01 compared to control.