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Secreted factors in conditioned media. Concentration of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and basic fibroblast growth factor (FGFb) in conditioned media at d2 and d5 measured using a Luminex bead-based multiplex assay. Obtained individual protein concentrations were normalized for sample loading by dividing by total protein in conditioned media. n = 4–8, ANOVA and Tukey’s posthoc test, *: p < 0.05, **: p < 0.001.
