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. 2018 Feb 9;9:160. doi: 10.3389/fmicb.2018.00160

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Copyright © 2018 Montalbán-López, Deng, van Heel and Kuipers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activity of the proteases in crude supernatants. Antimicrobial assay against L. lactis NZ9000 (pIL253 pNZ8048) using different proteases. Each protease (1 μl) was mixed with supernatant containing 50 μl of its corresponding prenisin variant. Activity observed results from protease activity on the substrate releasing active nisin. + indicates a positive control of 50 μl nisin 10 ng/μl. − indicates a negative control of untreated prenisin. Supernatants of induced L. lactis NZ9000 (pIL3BTC pNZE3nis-V8) (Glu-C), NZ9000 (pIL3BTC pNZE3nis-Thr) (Thrombin), NZ9000 (pIL3BTC pNZE3nisA-ASPR) (NisP-8H ASPR), NZ9000 (pIL3BTC pNZE3nisA-VSLR) (NisP-8H VSLR), NZ9000 (pIL3BTC pNZE3nis-Fx) (Factor Xa) were mixed with 1 μl of the specific protease.