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. 2018 Feb 9;9:160. doi: 10.3389/fmicb.2018.00160

Table 1.

Strains and vectors used in this work.

Strain Characteristics References
Lactococcus lactis NZ9000 nisRK::pepN Kuipers et al., 1997
Lactococcus lactis NZ9700 Nisin producer Kuipers et al., 1993
Micrococcus flavus B423 Sensitive strain NIZO Food Research
Escherichia coli Rosetta Blue DE3 Expression host Novagen
Plasmid Characteristics References
pET28b Vector with the IPTG inducible PT7. KmR Novagen
pNZ8048 CmR PnisA De Ruyter et al., 1996
pNZE3-empty EryR van Heel et al., 2013
pIL3BTC CmR PnisA-nisBTC Rink et al., 2005
pNZnisA-E3 EryR PnisA-nisA Kuipers et al., 2004
pNZE3-Cys-less C-terminal His-tagged Nisin (C7A C11A C18A C25A C27A) mutant. EryR Khusainov and Kuipers, 2013
pIL253 EryR Simon and Chopin, 1988
pNGnisTP CmR PnisA-nisTP Kuipers et al., 2004
pNZnisP-8H PnisA-nisP with 8 histidines tag after the subtilisin-like domain. CmR This work
pNZnisP-8K PnisA-nisP with 8 lysines tag after the subtilisin-like domain. CmR This work
pNZnisP-sol PnisA-nisP with no tag fused after the subtilisin-like domain. CmR This work
pNZnisPsl-8H PnisA-nisP sortase-less with 8 histidines tag. CmR This work
pNZnisPsl-8K PnisA-nisP sortase-less with 8 lysines tag. CmR This work
pETNisP-sol PT7-nisP with no tag fused after the subtilisin-like domain. KmR This work
pETNisP-8H PT7-nisP with 8 lysines tag after the subtilisin-like domain. KmR This work
pETnisP-8K PT7-nisP with 8 lysines tag fused after the subtilisin-like domain. KmR This work
pNZE3nisA-C7A-ASPR Nisin C7A mutant. EryR This work
pNZE3nisA-Cysless-ASPR Nisin (C7A C11A C18A C25A C27A) mutant lacking all cysteines. EryR This work
pNZE3nisA-CAAAA-ASPR Nisin (C11A C18A C25A C27A) mutant retaining only the first cysteine in the prepeptide. EryR This work
pNZE3nis-ringAless-ASPR Nisin (T2V S3A S5A C7A) mutant, EryR This work
pNZE3nisA-VSLR Nisin (A-4V P-2L) mutant containing a VSLR instead of the typical ASPR sequence in the leader, EryR This work
pNZE3nisA-C7A-VSLR Nisin (A-4V P-2L C7A) mutant with a VSLR NisP cleavage site, EryR This work
pNZE3nisA-Cysless-VSLR Nisin (A-4V P-2L C7A C11A C18A C25A C27A) mutant lacking all cysteines and with a VSLR NisP site, EryR This work
pNZE3nisA-CAAAA-VSLR Nisin (A-4V P-2L C11A C18A C25A C27A) mutant retaining only the first cysteine in the prepeptide and with a VSLR NisP site, EryR This work
pNZE3nisA-ringAless-VSLR Nisin (A-4V P-2L T2V S3A S5A C7A) mutant with a VSLR NisP site, EryR This work
pNZE3nisA-I1D Nisin I1D mutant, EryR This work
pNZE3nisA-I1W Nisin I1W mutant, EryR This work
pNZE3nisA-I1K Nisin I1K mutant, EryR This work
pNZE3nisA-T2K Nisin T2K mutant, EryR This work
pNZE3nisA-T2V Nisin T2V mutant, EryR This work
pNZE3-DDDK NisP cleavage site ASPR replaced by DDDK, EryR Plat et al., 2011
pNZE3-DDDDK NisP cleavage site GASPR replaced by DDDDK, EryR Plat et al., 2011
pNZE3-AFNLD Nisin D-19A mutant, EryR Plat et al., 2011
pNZE3-DANLD Nisin F-18A mutant, EryR Plat et al., 2011
pNZE3-DFALD Nisin N-17A mutant, EryR Plat et al., 2011
pNZE3-DFNAD Nisin L-16A mutant, EryR Plat et al., 2011
pNZE3-DFNLA Nisin D-15A mutant, EryR Plat et al., 2011
pNZE3-nis-V8 Nisin Z R-1E mutant, EryR This work
pNZE3-nis-Fx Nisin Z (A-4I S-3E P-2G) mutant with IEGR replacing the ASPR NisP cleavage site, EryR This work
pNZE3-nis-Thr Nisin Z (S-3V) mutant with AVPR replacing the ASPR NisP cleavage site, EryR This work
pNZE-nisΔ(23-34) Nisin Δ(23-34) deletion mutant, EryR Rink et al., 2007b
pNZE3-1765 nisin leader peptide fused to the leaderless part encoded by spr1765, EryR Majchrzykiewicz et al., 2010
pNZE3-1766 nisin leader peptide fused to the leaderless part encoded by spr1766, EryR Majchrzykiewicz et al., 2010

CmR, chloramphenicol resistance; EryR, erythromycin resistance.