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Kinetic characterization of the cleavage of several nisin leader peptide mutants.
| Prenisin variants | Km (μM) | Vmax (μmol/mg/min) | Kcat (s−1) | Kcat/Km (M−1s−1) |
|---|---|---|---|---|
| WT Prenisin (ASPR) | 0.73 ± 0.08 | 1.79 ± 0.10 | 1.25 ± 0.07 | 1.71 × 106 |
| Nis-Peng (VSLR) | 0.95 ± 0.08 | 1.79 ± 0.17 | 1.25 ± 0.11 | 1.32 × 106 |
| Thombin (AVPR) | 7.83 ± 0.27 | 3.39 ± 0.20 | 2.37 ± 0.14 | 3.03 × 105 |
| Factor Xa (IEGR) | 7.81 ± 0.01 | 3.32 ± 0.10 | 2.33 ± 0.07 | 2.98 × 105 |
Data indicate the mean value ± standard deviation.
