Table 1.
Half-maximal effective concentration (EC50) values of Photozipper (PZ) and the PZ variants, estimated from electrophoretic-mobility-shift assays with target sequence (Apo) and nonspecific (Cpo) double-stranded DNA
| Protein | Oligonucleotide | EC50 (nM) | ||
|---|---|---|---|---|
|
| ||||
| Dark | Light | Light-Dark | ||
| PZ | Apo | 420±50 | 39±1 | 410±40 |
| Cpo | 1,500±100 | 660±60 | 1,800±100 | |
|
| ||||
| PZ-S2C | Apo | 7.6±0.3 | 7.5±0.3 | ND |
| Cpo | 73±1 | 66±1 | ND | |
|
| ||||
| E159K | Apo | 490±40 | 61±2 | 410±40 |
|
| ||||
| E159R/K164R | Apo | 1,900±100 | 690±100 | 2,200±100 |
|
| ||||
| K164E | Apo | 1,200±100 | 850±60 | 1,200±100 |