FIGURE 2.

HrrS and ChrS target promoter profiling in response to heme. The C. glutamicum mutant strains ΔchrS or ΔhrrS were transformed with the target gene reporter pJC1_P_hmuO-eyfp or pJC1_P_hrtBA-eyfp, respectively. Cells were cultivated in a microbioreactor system (Biolector) in CGXII minimal medium with 2% (w/v) glucose containing 0–6 μM hemin. The maxima of the specific fluorescence after 7 h (P_hmuO, A and C) or 3 h (P_hrtBA, B and D) are shown. The specific fluorescence of mutant strains displaying background reporter levels was subtracted from the measurement (ΔhrrSA for P_hmuO-eyfp and ΔchrSA for P_hrtBA-eyfp). The reporter output over the time course of the experiment is shown in Supplementary Figure S2.