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. Author manuscript; available in PMC: 2018 Feb 14.
Published in final edited form as: J Cell Physiol. 2015 Sep 18;231(5):1001–1014. doi: 10.1002/jcp.25188

Fig. 4. Runx2 mRNA partitions equivalently in progeny of osseous cells following cell division.

Fig. 4

(A) Osteoblastic cell (hFOB) and osteosarcoma cells (U2OS, G292 and HOS) were subjected to RISH analysis. Scale bars: 20 µm, 100X oil objective, numerical aperture = 1.25. (B) Runx2 RISH signal intensity was determinede by quantitative image analysis in progeny cells at the last step of cytokinesis (n=10). Runx2 mRNA (RISH, left image) exhibits a partitioning coefficient equivalent to that of DNA (mitotic chromosomes staining, right image), demonstrating that this transcript segregates symmetrically in progeny cells upon cell division. Student’s t test was performed to assess the significance of the observed differences.