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. Author manuscript; available in PMC: 2018 Feb 14.
Published in final edited form as: Anal Chem. 2016 Oct 19;88(21):10347–10351. doi: 10.1021/acs.analchem.6b03350

Figure 2.

Figure 2

A) Overlay of the signal responses achieved for each concentration dilution of target miRNA cDNA product. Initial concentrations utilized were: 2 μM (black) , 1 μM (red) , 200 nM (blue) , 20 nM (pink) , 2 nM (green) , and a blank (purple) . B) Calibration curves for the HRP response of each miRNA target. The red curves represent linear fits of the initial slope of the HRP amplification step. Error bars represent the standard deviation of between 8 and 20 technical replicates at each concentration.