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. Author manuscript; available in PMC: 2018 Feb 14.
Published in final edited form as: Sci Signal. 2018 Jan 23;11(514):eaah4120. doi: 10.1126/scisignal.aah4120

Fig. 6. C-TSHR-mAb fails to activate major G proteins.

Fig. 6

(A) G protein activities were detected by dose-dependent luciferase activity assays in Chinese hamster ovary (CHO)–TSHR cells. The activities of the indicated G proteins (Gαs, Gαq, Gβγ, and Gα12) were measured after treatment of the cells with the indicated TSHR-mAbs (1 μg/ml) or TSH (1 mU/ml). Data are presented as the fold change in luciferase activity and are means ± SEM of three independent experiments. *P < 0.0001 compared to S-TSHR-mAb or TSH. (B to E) Dose-dependent activation or inhibition of the indicated G proteins by the indicated antibodies or TSH was determined by measurement of luciferase activity in the treated CHO-TSHR cells. Data are means ± SEM of three independent experiments.