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. Author manuscript; available in PMC: 2019 Jan 18.
Published in final edited form as: Nanoscale. 2018 Jan 18;10(3):1135–1144. doi: 10.1039/c7nr08188j

Figure 3.

Figure 3

(A) Cancer cell (U87MG) uptake of ferritin variants labeled with Cy5.5 fluorophore. Scale bar = 20 μm (B) Magnified confocal laser scanning imaging (CLSM) (Z-section) of intracellular uptake of mutant B ferritin after 2 h incubation. Scale bar = 10 μm. Blue: nucleus counterstained with DAPI; Red: Cy5.5 labeled mutant B ferritin; Green: F-actin stained with Alexa488 conjugated phalloidin. (C) Flow cytometry analysis of cellular uptake of mutant B ferritin at different concentrations. (D) MTT assay of mutant B ferritin with cancer cells (n = 6).