Skip to main content
. Author manuscript; available in PMC: 2018 Feb 14.
Published in final edited form as: Aging Cell. 2012 Oct 19;11(6):1065–1073. doi: 10.1111/acel.12008

Fig 5.

Fig 5

Polysomal assay in young (Y), middle-aged (M), and old (O) mice. Postnuclear preparations from the renal cortex of mice were separated into ten fractions on a 15–40% sucrose gradient, and mRNA for collagen type Iα2 was detected by RT–PCR. The percentage of total mRNA for collagen type Iα2 distributed to fractions 7–10 represents that bound to 80S ribosome and ready for translation; note the increment in this fraction of mRNA in the old mice. Mean data from two mice from each group are shown.