Table 1. MicroRNA levels in each of the four blood collection tubes from the comparison study.
Comparison of Ct values and SD values between the different sample types. Ct values for sample sets were defined as being suboptimal if mean microRNA expression values were ≥2 Cts greater than the set with the lowest Ct value. SD values for sample sets were defined as suboptimal if they were ≥2. For both Ct and SD assessment, samples that were not suboptimal were deemed to be satisfactory. Key to blood collection tubes: GSS = gel separator serum; EP = EDTA plasma; SDP = Streck DNA plasma; SRP = Streck RNA plasma.
| MicroRNA | Role | GSS | EP | SDP | SRP | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Ct value | SD | Conclusion | Mean Ct value | SD | Conclusion | Mean Ct value | SD | Conclusion | Mean Ct value | SD | Conclusion | ||
| cel-miR-39-3p | Exogenous normalization | 21.43 | 0.49 | Satisfactory | 21.13 | 0.85 | Satisfactory | 20.91 | 1.19 | Satisfactory | 25.23 | 4.29 | Suboptimal |
| hsa-miR-30b-5p | Endogenous normalization | 27.16 | 0.90 | Satisfactory | 29.57 | 1.18 | Suboptimal | 29.44 | 1.04 | Suboptimal | 34.41 | 4.45 | Suboptimal |
| hsa-miR-30c-5p | Endogenous normalization | 27.77 | 0.68 | Satisfactory | 29.93 | 1.16 | Suboptimal | 29.73 | 1.11 | Suboptimal | 33.78 | 4.16 | Suboptimal |
| hsa-miR-191-5p | Endogenous normalization | 25.91 | 0.72 | Satisfactory | 28.76 | 1.00 | Suboptimal | 30.02 | 1.49 | Suboptimal | 32.74 | 3.10 | Suboptimal |
| hsa-miR-23a-3p | Endogenous normalization (hemolysis) | 28.84 | 0.89 | Satisfactory | 30.26 | 1.10 | Satisfactory | 32.47 | 0.86 | Suboptimal | 39.20 | 0.84 | Suboptimal |
| hsa-miR-451a | Hemolysis assessment | 21.73 | 0.96 | Satisfactory | 21.55 | 0.72 | Satisfactory | 23.50 | 1.27 | Satisfactory | 32.90 | 4.30 | Suboptimal |