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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Immunol. 2018 Jan 8;200(4):1370–1381. doi: 10.4049/jimmunol.1700981

Figure 6.

Figure 6

Notch1 is a transcriptional target of E4bp4 in NK cells. (A) Notch1 locus showing location of predicted E4bp4 binding sites identified through the presence of the E4bp4 minimal consensus binding sequence TTA(T/C)(G/A)TAA(C/T) (Cowell, 2002). Filled boxes = exons; clear boxes = UTR regions. (B) ChIP analysis of E4bp4 binding at the Notch1 loci in chromatin from MNK-1 cells stably transduced with FLAG-E4bp4. E16 is a polyclonal antibody to E4bp4 and M2 is a monoclonal antibody to FLAG. Per2B was used as a positive control51 and gene desert as a negative control. Data is representative of three biological replicates. (C) QPCR analysis of Notch1 expression in Lin- BM cells. Data is representative of six biological replicates. Error bars show SEM. *, P < 0.05.