Table 3.
Comparison of regression curve parameters obtained with serotype/serogroup-specific qPCR using genomic DNA, NUversa and pNUversa as standards.
| Serotype/serogroup-specific qPCR | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 3 | 4 | 5 | 6ABCD | 7AF | 9VA | 14 | 18ABCF | 19F | 19A | 23F | Average | SD | bt-Test | ||
| Efficiency (%) | aChromosomal DNA | 96.6 | 96.4 | 98.1 | 101.2 | 98.5 | 100.1 | 98.2 | 91.2 | 99.0 | 101.1 | 91.6 | 96.0 | 97.3 | 3.247 | |
| NUversa linear | 93.5 | 100.9 | 94.2 | 100.7 | 91.7 | 94.1 | 107.5 | 100.0 | 95.7 | 95.6 | 94.8 | 95.8 | 97.0 | 4.417 | 0.859 | |
| pNUversa | 98.3 | 94.7 | 110.0 | 98.6 | 97.1 | 96.0 | 101.5 | 94.6 | 106.4 | 96.8 | 90.9 | 100.5 | 98.8 | 5.268 | 0.327 | |
| The coefficient of | aChromosomal DNA | 0.997 | 0.999 | 0.993 | 0.997 | 0.999 | 0.997 | 0.992 | 0.994 | 0.994 | 0.982 | 0.981 | 0.999 | 0.994 | 0.006 | |
| determination (R2) | NUversa linear | 0.997 | 0.999 | 0.998 | 0.998 | 0.990 | 0.993 | 0.987 | 0.982 | 0.966 | 0.997 | 0.988 | 0.999 | 0.991 | 0.010 | 0.438 |
| pNUversa | 0.991 | 0.999 | 0.992 | 1.000 | 0.994 | 0.992 | 0.999 | 0.997 | 0.995 | 0.997 | 0.995 | 0.999 | 0.996 | 0.003 | 0.298 | |
| Slope | aChromosomal DNA | –3.406 | –3.410 | –3.368 | –3.294 | –3.357 | –3.319 | –3.366 | –3.554 | –3.347 | –3.295 | –3.540 | –3.422 | –3.390 | 0.084 | |
| NUversa linear | –3.487 | –3.301 | –3.469 | –3.306 | –3.539 | –3.471 | –3.154 | –3.321 | –3.429 | –3.432 | –3.452 | –3.427 | –3.399 | 0.108 | 0.825 | |
| pNUversa | –3.362 | –3.457 | –3.103 | –3.357 | –3.393 | –3.422 | –3.287 | –3.458 | –3.178 | –3.402 | –3.562 | –3.310 | –3.358 | 0.126 | 0.353 | |
| y-intercept | aChromosomal DNA | 38.638 | 40.539 | 39.768 | 40.288 | 40.793 | 39.601 | 40.084 | 38.990 | 38.959 | 39.667 | 40.468 | 40.245 | 39.837 | 0.691 | |
| NUversa linear | 39.980 | 41.055 | 41.459 | 41.386 | 41.912 | 40.813 | 39.744 | 38.545 | 39.631 | 38.976 | 40.323 | 39.795 | 40.302 | 1.040 | 0.081 | |
| pNUversa | 42.008 | 44.527 | 42.262 | 44.369 | 43.615 | 43.144 | 42.894 | 41.956 | 41.524 | 41.889 | 44.880 | 42.240 | 42.942 | 1.157 | 2.49E-08 | |
a
Serotypes of strains for chromosomal DNA preps were 1, 3, 4, 5, 6A, 7F, 9V, 14, 18C, 19F, 19A and 23F
b
Set of parameter values from NUversa and pNUversa reactions were statistically compared to values from chromosomal DNA reaction with unpaired, two-tailed t-test.