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. 2017 Jul 10;17(5):fox048. doi: 10.1093/femsyr/fox048

Figure 4.

Figure 4.

Differential effect of short rapamycin treatment on Sch9 phosphorylation, trehalase activation and amino acids not solely transported by Gap1. TORC1 and PKA activation using cells of strain LW201 cells expressing Sch9–5HA (pJU676). (A) Inhibition of the appearance of Sch9 phosphorylated forms after addition of 25 mM L-glutamine by treatment with rapamycin for 8 min. Representative result of three repeats. (B-D) Trehalase activation after addition of 1 mM L-glutamine, L-arginine or L-leucine without (closed circles) or with (open cirlces) 8 min pre-incubation with rapamycin. Representative result of two repeats. (E-I) Uptake of 1 mM L-glutamine, L-arginine, L-leucine, L-citrulline or L-proline after pre-incubation with rapamycin for 8 or 30 min. Representative result of two repeats.