Figure 3.

Early biofilms of V. cholerae A1552 exposed to T. pyriformis for 72 h. Biofilm biomass was determined by CV staining (A) and the planktonic cells in the supernatant enumerated by the drop plate method (B). Data were natural log transformed and the percentage change in biofilm biomass was calculated by removing the ungrazed biomass from the grazed biofilm biomass divided by the ungrazed. The experiment was run in three replicates and repeated three times separately. Error bars represent standard deviation. Statistical analysis was performed using Student's t-test that revealed no significant difference, P = 0.3 (A) and P = 0.1 (B).