Figure 4.

Chronological lifespan is affected by the loss of storage metabolism. The Y. lipolytica wild-type strain (WT) and its derivatives deleted for glycogen (gly⁻) or neutral lipid (NL⁻) synthesis or both (NL⁻ gly⁻) were cultivated until depletion of glucose (C-lim minimal medium), and the survival rate was determined by plating diluted samples. The viability of the NL-deficient strain was strongly reduced in comparison to its parent and the additional deletion of YlGSY1 in this mutant, resulting in a strain without the ability to store carbon, caused a further reduction of the survival rate.