Table 2.

Physiological data for wild-type and deletion mutant in nitrogen-limited media.

	YlWT			Ylgsy1Δ
	24 h	48 h	72 h	24 h	48 h	72 h
I. Glucose
Biomass (g L−1)
CDW	2.4 ± 0.2	3.4 ± 0.2	3.5 ± 0.1	2.0 ± 0.1	3.2 ± 0.2	3.4 ± 0.1
Extracellular metabolites (g L−1)
Glucose	14.7 ± 0.6	9.4 ± 0.4	6.2 ± 0.3	15.4 ± 0.3	10.1 ± 0.1	6.9 ± 0.1
Citrate	n.d.	0.4 ± 0.1	1.3 ± 0.2	n.d.	0.1 ± 0.0	1.1 ± 0.1
Succinate	0.2 ± 0.0	0.2 ± 0.0	n.d.	0.2 ± 0.0	0.2 ± 0.0	n.d.
Mannitol	0.5 ± 0.2	1.6 ± 0.3	2.2 ± 0.3	0.4 ± 0.1	1.3 ± 0.1	1.7 ± 0.1
Intracellular metabolites (mg gCDW−1)
Trehalose	0.33 ± 0.14	0.09 ± 0.05	0.10 ± 0.02	0.06 ± 0.04	n.d.	0.02 ± 0.00
Glycogen	123 ± 9	84 ± 2	44 ± 2	n.d.	n.d.	n.d.
TAG	110 ± 18	221 ± 21	204 ± 6	179 ± 21*	263 ± 11*	242 ± 26
II. Glycerol
Biomass (g L−1)
CDW	2.6 ± 0.1	3.8 ± 0.0	4.0 ± 0.1	2.2 ± 0.1	3.3 ± 0.1	3.6 ± 0.1
Extracellular metabolites (g L−1)
Glycerol	14.7 ± 0.8	7.7 ± 0.4	2.5 ± 0.3	14.9 ± 0.7	6.2 ± 0.4	n.d.
Citrate	n.d.	0.9 ± 0.1	2.1 ± 0.4	n.d.	1.3 ± 0.1	3.2 ± 0.7
Succinate	0.1 ± 0.1	0.1 ± 0.1	0.1 ± 0.0	0.1 ± 0.1	0.1 ± 0.1	n.d.
Mannitol	0.4 ± 0.0	2.9 ± 0.5	5.1 ± 0.9	0.7 ± 0.1	3.7 ± 0.5	6.2 ± 0.5
Intracellular metabolites (mg gCDW−1)
Trehalose	0.60 ± 0.11	0.29 ± 0.02	0.18 ± 0.03	0.13 ± 0.06	0.03 ± 0.00	0.01 ± 0.01
Glycogen	141 ± 8	165 ± 16	119 ± 13	n.d.	n.d.	n.d.
TAG	104 ± 28	149 ± 13	152 ± 16	167 ± 31	260 ± 20*	248 ± 18*

n.d., not detected

^* P ≤ 0.05 in unpaired two-tailed t-tests