Table 1.

List of selected web-based bioinformatics tools for gRNA design in yeast.

Name	Link	Reference	Input	Main features	Yeast species
CRISPy	http://staff.biosu stain.dtu.dk/laeb/ crispy_yeast/	Ronda et al. (2014 ); Jakočiūnas et al. (2015a)	Gene name/ID	Off-target	S. cerevisiae reference, CEN.PK
CRISPy-web	http://crispy.second arymetabolites.org	Blin et al. (2016)	Gene name/ID, genomic coordinates	Off-target	Any user-submitted genome
CRISPR-ERA	http://crispr- era.stanford.edu/	Liu et al. (2015)	Gene name, genomic coordinates, sequence	Off-target, efficiency score, gene repression/activation	S. cerevisiae reference
CHOPCHOP v2	http://chopchop.cbu. uib.no	Labun et al. (2016)	Gene name, genomic coordinates, sequence	Off-target user defined, on-target algorithm, restriction sites	S. cerevisiae reference,
					C. albicans,
					C. tropicalis,
					C. glabrata,
					P. pastoris
CRISPRdirect	https://crispr. dbcls.jp/	Naito et al. (2015)	Gene name, genomic coordinates, sequence	Off-target, GC content, poly T, restriction sites	S. cerevisiae,
					Sch. pombe,
					K. lactis,
					Y. lipolytica,
					C. albicans,
					C. glabrata
E-CRISPR	http://www.e- crisp.org/	Heigwer, Kerr and Boutros (2014)	Gene symbol, sequence	Off-target, on-target algorithm, gene activation/repression	S. cerevisiae,
					Sch. pombe
Yeastriction	http://yeastriction. tnw.tudelft.nl	Mans et al. (2015)	Gene name	Off-target, AT content, self-complementarity, restriction sites	S. cerevisiae, several strains
Benchling	https://benchling. com/ crispr		Gene name, coordinates, sequence	Off-target, on-target algorithm	S. cerevisiae reference,
					Sch. pombe,
					C. albicans,
					Y. lipolytica
ATUM gRNA Design Tool	https://www.atum. bio/eCommerce/ cas9/input		Gene name, coordinates, sequence	Off-target	S. cerevisiae reference