Table 1.
Potential strategies for single-cell identification and targets of interest. Numbering corresponds to labeling targets in Figure 2.
| Target | Example |
|---|---|
| Labeling strategies | |
| (1) Extracellular integration | Oligosaccharide labeling (Geva-Zatorsky et al.2015) |
| (2) Extracellular recognition | DNA aptamer (Chang et al.2013), phage binding domain (Kretzer et al.2007) |
| (3) Extracellular enzyme affinity | Fluorescent substrate (Martinez-Garcia et al.2012), glycoside hydrolases (Chauvigne-Hines et al.2012), phytase (Berry and Harich 2013), penicillin binding proteins (Kocaoglu and Carlson 2013), β-lactamases (Bottcher and Sieber 2012; Shao and Xing 2012), showdomycin (Bottcher and Sieber 2010) |
| (4) Intracellular receptor | Quorum sensing (Mukherji et al.2013) |
| (5) Intracellular product | Polyhydroxybutyrate granules (Tyo, Zhou and Stephanopoulos 2006), hypochlorous acid production (Sun et al.2014), ROS production (Gomes, Fernandes and Lima 2005) |
| (6) Intracellular enzymatic reaction | Non-ribosomal peptide synthetases (Konno et al.2015), polyketide synthases (Meier et al.2009), nucleophilic RNAs (McDonald et al.2014), adenylation (Duckworth et al.2012), glyceraldehyde-3-phosphate dehydrogenase (Kaschani et al.2012), glutathione reductase (Lou et al.2014), sulfatase (Park, Rhee and Hong 2012), phosphatase (Kim et al.2011), aldehyde dehydrogenase (Adam, Cravatt and Sorensen 2001), palmitoyl actytransferases (Zheng et al.2013), β-glucosidases (Kallemeijn et al.2012; Walvoort et al.2012), agmatine deiminase (Marchenko et al.2015), monoamine oxidase (Li et al.2014), N-acetylgalactosaminidase (Kalidasan et al.2013), alkaline phosphatase (Kim et al.2011), metalloproteases (Sieber et al.2006), cysteine proteases (Kato et al.2005), serine hydrolase (Liu, Patricelli and Cravatt 1999) |
| (7) Intracellular integration | BONCAT (Dieterich et al.2007), degenerate tRNA synthatases (Ngo et al.2009), RNA labeling (Paredes and Das 2011) |
| (8) Isotope integration | 2H2O (Berry et al.2015), 13CO2 (Li et al.2012), [13C] naphthalene (Huang et al.2009) |
| Characteristics | |
| General properties | Magnetotaxis (Kolinko et al.2012), cell morphology (Marcy et al.2007) |
| Cellular activity | Electron transport chain (Kaprelyants and Kell 1993), live/dead (Nocker, Cheung and Camper 2006), reductase (Lahtinen et al.2008) |
| Intracellular ions | pH (Han and Burgess 2010), [Zn2+] (Walkup et al.2000) |