Fig 3. Effect of anti-LIF antibody on migration and invasion of HSC3 cells.

Transwell migration (A) and invasion (B) assays performed using cell-culture inserts. In the upper chamber, 2×10⁴ HSC3 cells were seeded, and 1×10⁵ NHDFs were seeded into the lower well. In each experiment, cells were treated with 0.5 μg/mL of an anti-LIF neutralizing antibody. IgG was used as a control. After 48 h, the migrated or invaded HSC3 cells on the lower surface of the upper chamber were stained and quantified. Representative images and graphs of 3 independent experiments are shown. Multiple comparisons were performed by using one-way ANOVA with Tukey’s method. *p < 0.05, **p < 0.01 compared with control NHDFs. Data represent means ± SEM.