Table 1. TPM value and ranking level of lineage-specific genes in hESC lines H7, HUES1, HUES8 and HUES9.
Lineage | Marker | TPM | Ranking | ||||||
---|---|---|---|---|---|---|---|---|---|
H7 | HUES1 | HUES8 | HUES9 | H7 | HUES1 | HUES8 | HUES9 | ||
hESCs | POU5F1 | 861.66 | 414.30 | 460.03 | 176.94 | 86 | 256 | 219 | 826 |
SOX2 | 317.49 | 654.37 | 634.96 | 433.28 | 347 | 134 | 135 | 228 | |
PODXL | 1322.01 | 1115.44 | 1087.66 | 898.01 | 49 | 55 | 61 | 75 | |
CDH1 | 116.19 | 183.08 | 226.13 | 131.61 | 1362 | 779 | 593 | 1232 | |
THY1 | 383.97 | 107.21 | 104.67 | 71.72 | 260 | 1605 | 1677 | 2756 | |
EPCAM | 171.07 | 212.11 | 199.24 | 119.70 | 811 | 653 | 717 | 1416 | |
CD9 | 116.48 | 52.72 | 53.88 | 27.92 | 1356 | 3792 | 3735 | 7452 | |
ITGB1 | 131.23 | 120.19 | 113.06 | 120.75 | 1159 | 1390 | 1523 | 1397 | |
CD59 | 33.52 | 31.81 | 32.66 | 25.71 | 5628 | 6275 | 6192 | 7923 | |
PROM1 | 31.34 | 44.75 | 44.14 | 38.73 | 5977 | 4514 | 4638 | 5591 | |
PREX1 | 38.68 | 28.22 | 28.71 | 72.21 | 4927 | 6938 | 6848 | 2733 | |
SALL4 | 142.81 | 273.96 | 284.41 | 305.08 | 1033 | 436 | 424 | 366 | |
MYC | 67.37 | 45.14 | 48.58 | 18.39 | 2701 | 4466 | 4198 | 9807 | |
TDGF1 | 157.48 | 116.31 | 131.87 | 48.52 | 907 | 1455 | 1212 | 4388 | |
DNMT3B | 640.81 | 715.49 | 888.24 | 533.03 | 131 | 112 | 82 | 164 | |
ALPL | 266.77 | 407.57 | 369.04 | 336.52 | 436 | 261 | 307 | 325 | |
TDGF1 | 157.48 | 116.31 | 131.87 | 48.52 | 907 | 1455 | 1212 | 4388 | |
Mesendoderm | MIXL1 | 8.18 | 3.22 | 2.17 | 1.31 | 12617 | 16066 | 17066 | 18297 |
GSC | 3.55 | 2.36 | 3.34 | 2.05 | 15474 | 16882 | 15976 | 17616 | |
Mesoderm | T | 2.10 | 2.29 | 0.87 | 1.30 | 16832 | 16951 | 18558 | 18306 |
CD34 | 0.52 | 0.90 | 1.68 | 1.40 | 18707 | 18509 | 17601 | 18198 | |
GATA4 | 0.70 | 0.84 | 2.31 | 0.63 | 18476 | 18570 | 16925 | 18951 | |
HAND1 | 0.18 | 1.49 | 4.68 | 4.38 | 19186 | 17827 | 15010 | 15858 | |
TWIST2 | 5.81 | 3.08 | 1.12 | 4.27 | 13958 | 16202 | 18249 | 15932 | |
GATA6 | 1.04 | 1.25 | 2.33 | 0.83 | 18041 | 18099 | 16898 | 18724 | |
Endoderm | CXCR4 | 5.28 | 10.20 | 11.27 | 29.13 | 14254 | 12112 | 11620 | 7204 |
SOX17 | 1.73 | 1.74 | 3.25 | 1.22 | 17196 | 17545 | 16063 | 18371 | |
FOXA2 | 1.72 | 1.07 | 2.90 | 0.72 | 17205 | 18304 | 16363 | 18853 | |
AFP | 0.36 | 0.32 | 3.48 | 0.71 | 18930 | 19219 | 15863 | 18872 | |
AMN | 20.35 | 19.57 | 19.89 | 21.70 | 8277 | 8869 | 8788 | 8880 | |
SOX7 | 10.48 | 9.34 | 10.41 | 7.75 | 11547 | 12483 | 11968 | 13900 | |
HNF4A | 0.29 | 0.20 | 0.85 | 0.20 | 19036 | 19301 | 18583 | 19288 | |
FOXA1 | - | - | - | - | - | - | - | - | |
Ectoderm | PAX6 | 0.99 | 13.92 | 19.80 | 40.43 | 18102 | 10658 | 8812 | 5356 |
TUBB3 | 36.09 | 32.00 | 24.20 | 24.45 | 5271 | 6235 | 7768 | 8203 | |
SOX1 | 3.42 | 7.46 | 4.60 | 8.28 | 15597 | 13391 | 15063 | 13638 | |
FGF5 | - | - | - | - | - | - | - | - |