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. Author manuscript; available in PMC: 2018 Feb 14.
Published in final edited form as: J Am Chem Soc. 2017 Jun 20;139(25):8384–8387. doi: 10.1021/jacs.7b00794

Figure. 2. Hydroxylation at ProB28 modulates insulin dimerization, dissociation kinetics, and stability.

Figure. 2

(A) Far UV CD spectra collected on 60 µM insulins in 10 mM phosphate buffer, pH 8.0 at 25°C. (B) Insulin hexamer dissociation following sequestration of Zn2+ by terpyridine. Zn2+-(terpy) signal was monitored at 334 nm and fitted to a mono-exponential decay. HzpI and HypI contain 10% ProI. The curves for HypI and ProI are indistinguishable in this plot. (C) Representative fibrillation curves for 60 µM insulins (37°C, 960 RPM; n=4). Insulin fibrils were detected by the rise in Thioflavin T (ThT) fluorescence that accompanies binding to fibrillar aggregates.