Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers |
|---|---|---|---|
| strain, strain background (E.coli K12 AB1157) |
mukBmYPetEF | SN 182 Nolivos et al.; DOI:10.1038/ncomms10466 |
|
| strain, strain background (E.coli K12 AB1157) |
mukB EQ mYPetEF | SN 182 Nolivos et al.; DOI:10.1038/ncomms10466 |
|
| Strain, for protein expression (E.coli C3013I) |
NEB | ||
| strain, for MukB protein expression (E.coli C3013I) C3013I - mukB 3xflag tag |
mukB 3xFLAG C3031 | FLOI - this work; derivative of SN 54; Nolivos et al; DOI:10.1038/ncomms10466 |
|
| strain, strain background (E.coli K12 AB1157) |
RRL 149; Δ mukB | Nolivos et al.; DOI:10.1038/ncomms10466 |
|
| strain, strain background (E.coli K12 AB1157) |
Ab 233; Δ mukF mukBGFP | Nolivos et al.; DOI:10.1038/ncomms10466 |
|
| antibody | anti MukF- (mouse polyclonal) | gift from Kenneth Marians, Memorial Sloan Kettering Center, New York |
|
| antibody | Anti-Mouse IgG (whole molecule)– Peroxidase antibody produced in goat |
SIGMA | RRID: AB_258167 |
| commercial assay or kit | ENZCheck Phosphate Assay | Life Technologies | |
| software, algorithm | ASTRA 6 | Wyatt Technologies | |
| software algorithm | MicrobTracker-Matlab | Sliusarenko et al. (2011) | RRID:SCR_001622 |
| software, algorithm | Pymol | https://pymol.org/2/ | RRID:SCR_000305 |
| software, algorithm | Methamorph - Ni elements | Nikon | |
| software, algorithm | Modeller | RRID:SCR_008395 | |
| software algorithm | MARS data analysis | BMG Lagtech |