. Author manuscript; available in PMC: 2018 Dec 27.

Published in final edited form as: J Am Chem Soc. 2017 Dec 15;139(51):18409–18427. doi: 10.1021/jacs.7b08418

Table 1.

Kinetic parameters of SLO and mutants in 0.1 M borate (pH 9) at 30°C

Enzyme	k_cat (s⁻¹)	E_a(H)^f (kcal/mol)	^Dk_cat^g	ΔE_a^h (kcal/mol)	R₀ⁱ	Ωⁱ
WT^a	297 (12)	2.1 (0.2)	81 (5)	0.9 (0.2)	2.85	319.9
I553V^b	91 (5)	2.4 (0.5)	77 (6)	2.6 (0.5)	2.96	271.0
I553L^b	273 (10)	0.4 (0.7)	81 (3)	3.4 (0.6)	3.073	243.2
I553A^a	280 (10)	1.9 (0.2)	93 (4)	4.0 (0.3)	3.043	251.5
I553G^b	58 (4)	0.03 (0.04)	178 (16)	5.3 (0.7)	3.317	217.9
L546A^a	4.8 (0.6)	4.1 (0.4)	93 (9)	1.9 (0.6)	--	--
L754A^a	0.31 (0.02)	4.1 (0.3)	112 (11)	2.0 (0.5)	--	--
L546A/L754A (DM)^c	0.025 (0.01)	9.9 (0.2)	692 (43)	0.3 (0.7)	See Figure 2
L546A/I553A^d	2.21 (0.09)	3.8 (0.4)	128 (3)	2.8 (0.4)	--	--
L754A/I553A^d	0.56 (0.03)	6.9 (0.2)	85 (7)	3.9 (0.5)	--	--
Y317L^e	267 (8)	1.0 (0.1)	68 (2)	2.3 (0.2)	--	--

From Ref ⁴².

From Ref ⁴⁴.

The values of k_cat, ^Dk_cat and ΔE_a are From Ref ⁵¹, while the E_a(H) value is from Ref ⁵³.

From Ref ⁵⁵.

From Ref ⁸¹.

Determined from the temperature dependence of k_cat.

^gD

k_cat=k_cat(H)/k_cat(D).

ΔE_a=E_a(D) -E_a(H).

ⁱ

These are computed according to the vibronically non-adiabatic multi-dimensional model, Ref ⁵¹.