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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Atherosclerosis. 2017 Dec 9;269:71–78. doi: 10.1016/j.atherosclerosis.2017.12.016

Figure 4. Regulation of p27Kip1 by the mTOR pathway is lost under diabetic conditions.

Figure 4

(A) Representative Western blots for p27Kip1, β-actin, and 4E-BP1 in ND and DM mice undergoing femoral artery wire injury, coupled with intraperitoneal injection of either vehicle or rapamycin. The contralateral artery underwent a sham procedure. (B) Densitometry of Western blots for p27Kip1 for ND (n=4 for vehicle, n=6 for rapamycin) and DM (n=3 for both groups) mice. Data is presented as mean of the percentage of p27Kip1 in the injured artery compared to the sham. This results in a reduction of the number of comparisons to 6. (C) Representative Western blots for p27Kip1 and β-actin in ND-VSMCs and DM-VSMCs. VSMCs were serum starved overnight (U), stimulated with DMEM/20% FBS supplemented with the indicated dose of rapamycin (RPM). (D) Densitometry of Western blots for p27Kip1 for ND-VSMCs and DM-VSMCs treated as indicated in C. All studies were performed in triplicate. Bar p < 0.05. (E) p27Kip1 protein levels in the aortae of ND and DM mice (n=4 for both groups) as measured by ELISA assay. † p < 0.05. (F) p27Kip1 mRNA in the aortae of ND and DM mice (n=3 for both groups).