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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Atherosclerosis. 2017 Dec 9;269:71–78. doi: 10.1016/j.atherosclerosis.2017.12.016

Figure 5. Increased ERK-1/2 activity promotes increased miR-221 and -222 as well as p27Kip1 mRNA destabilization.

Figure 5

(A) Ratio of phosphorylated to total ERK-1/2 in the aortae of DM (n=3) and ND (n=4) mice as measured by ELISA. Bar p < 0.05. (B) Half-life of p27Kip1 mRNA in ND-VSMCs and DM-VSMCs treated with vehicle (Veh) and PD98059 (PD). Data are presented as mean of triplicate samples ± standard error of the mean. † p < 0.05. (C) Half-life of p27Kip1 mRNA in ND-VSMCs and DM-VSMCs transfected with non-targeting control oligonucleotides (NT) or siRNA targeting ERK 1/2 (ERK siRNA). Data are presented as mean of triplicate samples ± standard error of the mean. † p < 0.05. (D) Relative levels of miR-221 and -222 in serum stimulated ND-VSMCs and DM-VSMCs treated with vehicle or U0126 (50 µmol/L) overnight. Data are normalized to the vehicle treated samples. Bar p < 0.05. (E) Relative p27Kip1 mRNA levels in the aortae of ND mice treated with vehicle (ND, n=3) or U0126 (ND+EI, n=5) and DM mice treated with vehicle (DM, n=4) or U0126 (DM+EI, n=4). Data are normalized to ND. Bar p < 0.05. (F) Relative p27Kip1 protein levels in the aortae of ND mice treated with vehicle (ND, n=4) or U0126 (ND+EI, n=4) and DM mice treated with vehicle (DM, n=4) or U0126 (DM+EI, n=6). Bar p < 0.05.