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. 2018 Feb 14;84(5):e02274-17. doi: 10.1128/AEM.02274-17

TABLE 2.

Chemical composition, fermentation products, and mycotoxin concentration of purple prairie clover ensiled without or with polyethylene glycol in terminal silage and after 7 and 14 days of aerobic exposure

Item Terminal silage (n = 5)
Aerobic exposure silage (n = 4)
Day 0
SEM P Day 7
SEM P Day 14
SEM P
Control PPC-p Control PPC-p Control PPC-p
Chemical composition (g/kg DM)
    Dry matter (g/kg forage) 305.94 311.76 4.77 0.375 387.51 388.96 5.10 0.848 376.59 389.44 3.80 0.054
    Organic matter 911.34 910.25 1.40 0.599 912.56 913.64 0.70 0.383 902.89 901.93 2.94 0.826
    Neutral detergent fiber 437.43 428.28 13.55 0.637 437.11 438.40 4.65 0.850 527.00 509.77 20.08 0.545
    Acid detergent fiber 357.21 366.93 8.27 0.430 369.04 380.78 5.76 0.199 442.74 420.33 13.06 0.292
    Acid detergent-insoluble nitrogen 9.03 7.76 0.13 <0.001 8.85 6.32 0.12 <0.001 11.26 10.26 1.36 0.658
    Neutral detergent-insoluble nitrogen 7.45 8.21 0.44 0.270 11.92 10.30 0.60 0.095 13.06 13.32 1.06 0.878
    Acid detergent lignin 70.56 82.09 9.17 0.400 103.07 98.62 6.29 0.622 148.78 132.49 8.60 0.252
    Water-soluble carbohydrates 2.40 0.30 0.12 <0.001 0.69 0.47 0.06 0.034 0.51 0.42 0.09 0.482
    Total N 28.16 28.24 0.06 0.457 28.62 28.32 0.18 0.363 27.82 28.45 0.83 0.569
    Soluble N 0.29 0.45 0.02 <0.001 0.26 0.45 0.02 <0.001 0.22 0.42 0.02 <0.001
    Nonprotein N 0.35 0.50 0.02 <0.001 0.24 0.38 0.01 <0.001 0.16 0.35 0.02 <0.001
    Extractable condensed tannins 27.4 ± 1.5a NDb 4.0 ± 0.5a ND ND ND
Fermentation products (g/kg DM)
    pH 5.02 4.61 0.01 <0.001 4.98 4.66 0.02 <0.001 8.81 8.96 0.18 0.586
    Lactic acid 40.99 91.04 5.36 <0.001 27.13 63.48 2.57 <0.001 1.92 3.14 0.40 0.161
    Total VFA 15.59 26.84 1.70 0.002 22.38 36.12 2.55 0.009 1.85 1.60 0.46 0.730
    Acetic acid 14.82 25.38 2.68 0.022 21.78 33.93 2.58 0.016 1.14 0.88 0.32 0.635
    Propionic acid 0.17 0.97 0.06 <0.001 0.17 1.59 0.12 <0.001 0.05 0.10 0.02 0.184
    Butyric acid 0.38 0.36 0.01 0.408 0.33 0.41 0.05 0.291 0.29 0.34 0.01 0.087
    Minor VFAc 0.28 0.17 0.04 0.101 0.12 0.16 0.01 0.087 0.26 0.30 0.04 0.539
    Succinic acid 5.54 2.60 0.39 <0.001 3.72 1.84 0.32 0.006 1.20 0.66 0.48 0.453
    Lactic acid:acetic acid ratio 2.49 3.62 0.18 0.003 1.28 1.80 0.17 0.067 1.09 3.60 0.20 0.001
    Ammonia N 2.70 3.74 0.14 <0.001 2.10 3.26 0.18 0.004 2.13 4.22 0.25 0.001
Mycotoxin concn (μg/kg DM)
    Deoxynivalenol 108.6 190.9 3.93 0.004 150.2 186.4 2.31 0.008 474.7 679.4 10.92 0.006
    Ochratoxin A 12.0 44.0 5.29 0.003 13.67 39.51 3.34 0.009 25.60 71.64 1.68 0.003
a

Values = mean ± standard error.

b

ND, not detectable.

c

Minor VFA is isobutyric acid + valeric acid + isovaleric acid + caproic acid.