FIG 1.

Biofilm formation and release of extracellular DNA (eDNA) by wild-type C. jejuni strains (i.e., human 10, 81-116, ATCC 33560, 87-95, NCTC 11168, 1658, and F38011) under optimal, aerobic, and starvation conditions. (A) The level of biofilm formation was evaluated using crystal violet staining. The stained biofilm was released using 95% ethanol and determined by monitoring the value of OD₅₉₅. (B to D) The concentration of eDNA during biofilm formation under optimal conditions (B), aerobic conditions (C), and starvation conditions (D) over 3 days was quantified using SYBR green I dye on the basis of a standard curve generated using a series of 10-fold dilutions of Lambda DNA from 80 μg/ml to 0.156 μg/ml.