FIG 6.

DNase I treatment before bacterial initial attachment prevented C. jejuni biofilm formation and DNase I treatment of the well-developed C. jejuni biofilm disrupted biofilm structure, leading to the reduction of biomass. To treat biofilm at the initial attachment stage, DNase I was mixed with C. jejuni culture to reach a final concentration of 2 U/ml and then added into the 96-well plate for biofilm formation. To treat the well-developed biofilm in the 96-well plate, 200 μl of DNase I solution (2 U/ml) was added into a well with a 3-day cultivated C. jejuni biofilm. The treatment was maintained for 15 min at room temperature. The reduction level of biofilm was evaluated using the aforementioned crystal violet staining assay. Asterisks denote significant difference (P < 0.05).