FIG 4.

Conjugative transfer of ICESt3 from and/or to mutants. Conjugation frequencies of LMG 18311 mutants relative to the WT are shown. (A) Conjugative transfer of ICESt3 using a WT donor and a mutated recipient; (B) conjugative transfer of ICESt3 using a mutated donor and a WT recipient; (C) conjugative transfer of ICESt3 using a mutated donor and a mutated recipient. For each experiment, means and standard deviations from at least 3 biological repetitions on 2 independent clones of mutants are shown. * and **, the conjugation frequency is statistically different from that of the WT (P < 0.05 and P < 0.01, respectively [t test]).