TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| LMG 18311 | Wild-type strain | |
| LMG 18311(ICESt3) | LMG 18311 carrying ICESt3 tagged with a chloramphenicol resistance cassette; Cmr | 11 |
| LMG 18311(ICESt3 GFP) | LMG 18311 carrying ICESt3 tagged with a chloramphenicol resistance cassette and with a GFP gene under the control of the pLDH promoter of S. thermophilus LMG 18311; Cmr | This work |
| LMG 18311 Eryr | LMG 18311 carrying an erythromycin resistance cassette in its chromosome between stu0627 and stu0629 genes; Eryr | This work |
| LMG 18311 Spcr | LMG 18311 carrying a spectinomycin resistance cassette in its chromosome between stu0627 and stu0629 genes; Spcr | This work |
| LMD-9(pMG36e) | LMD-9 carrying the pMG36e plasmid conferring erythromycin resistance; Eryr | This work |
| Mutated recipient cells for mating with WT donor cells | ||
| LMG 18311 ΔlspA | LMG 18311 with lspA (stu0521) gene deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 ΔtagO-like | LMG 18311 with tagO-like (stu0163) gene deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 ΔdltA-like | LMG 18311 with dltA (stu0761) gene deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 ΔyfnI-like | LMG 18311 with yfnI-like (stu0636) gene deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 ΔmprF-like | LMG 18311 with mprF-like (stu1256) gene deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 ΔepsE | LMG 18311 with epsE (stu1108) gene deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 Δeps9 Δeps10 Δeps11 | LMG 18311 with eps9 to eps11 (stu1097 to stu1099) genes deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMG 18311 Δstu1482 ΔrgpX2 | LMG 18311 with stu1482 and rgpX2 (stu1482 and stu1473) genes deleted by insertion of an erythromycin cassette; Eryr | This work |
| LMD-9 ΔsrtA | LMD-9 with srtA gene deleted by insertion of an erythromycin cassette; Eryr | 43 |
| Mutated donor cells for mating with WT recipient cells | ||
| LMG 18311(ICESt3) ΔlspA | LMG 18311 ΔlspA carrying ICESt3; Eryr Cmr | This work |
| LMG 18311(ICESt3) ΔtagO-like | LMG 18311 ΔtagO-like carrying ICESt3; Eryr Cmr | This work |
| LMG 18311(ICESt3) ΔdltA-like | LMG 18311 ΔdltA-like carrying ICESt3; Eryr Cmr | This work |
| LMG 18311(ICESt3) ΔyfnI-like | LMG 18311 ΔyfnI-like carrying ICESt3; Eryr Cmr | This work |
| LMG 18311(ICESt3) ΔmprF-like | LMG 18311 ΔmprF-like carrying ICESt3; Eryr Cmr | This work |
| LMG 18311(ICESt3) ΔepsE | LMG 18311 ΔepsE carrying ICESt3; Eryr Cmr | This work |
| LMG 18311(ICESt3) Δeps9 Δeps10 Δeps11 | LMG 18311 Δeps9 Δeps10 Δeps11 carrying ICESt3; Eryr Cmr | This work |
| Mutated recipient cells used in mutant/mutant experiments | ||
| LMG 18311 ΔlspA | LMG 18311 ΔlspA with spectinomycin resistance cassette; Eryr Spcr | This work |
| LMG 18311 ΔtagO-like | LMG 18311 ΔtagO-like with spectinomycin resistance cassette; Eryr Spcr | This work |
| LMG 18311 ΔyfnI-like | LMG 18311 ΔyfnI-like with spectinomycin resistance cassette; Eryr Spcr | This work |
| LMG 18311 ΔepsE | LMG 18311 ΔepsE with spectinomycin resistance cassette; Eryr Spcr | This work |
| Plasmids | ||
| pMG36e | 3.4 kb, replication origin from pWV01; Eryr | 44 |
| pG+host9 | 4.6 kb, thermosensitive derivative of pWV01; Eryr | 45 |
| pSL1180 spec lox | 4.5 kb, derivative of pBR322; Spcr | This work |
| pSET4s | 4.5 kb, thermosensitive derivative of pWV01; Spcr | 46 |
| pOri23 | 5.8 kb, ColE1 replication origin; Eryr | 47 |
| pOri23-pLDH | pOri23 with the promoter of the l-lactate dehydrogenase gene (stu1280) of S. thermophilus LMG18311 instead of the P23 promoter | This work |
| pSW4-GFPopt | 7.3 kb, Gram-positive/Gram-negative shuttle vector with GFPopt gene; Kmr Apr | 42 |
| pOri23-pLDH-gfp | pOri23 vector carrying the GFP gene of pSW4-GFPopt under the control of the pLDH promoter of S. thermophilus LMG18311 | This work |
a
Abbreviations: Cmr, chloramphenicol resistance; Eryr, erythromycin resistance; Spcr, spectinomycin resistance; Kmr, kanamycin resistance; Apr, ampicillin resistance.