Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 14;8:2996. doi: 10.1038/s41598-018-20448-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Neuronal morphology is not affected by knocking down VPS35 in neurons. (a,b) Representative confocal microscopy images of hippocampal autaptic neurons containing either control shRNA or one of the three shRNAs against VPS35. Scale bar = 50 μm. (a) Immunolabelling for MAP2 and SMI-312. Merge image of the MAP2 (green) SMI-312 (magenta). (b) Immunolabelling for VAMP2/synaptobrevin, Synaptophysin and Bassoon. Quantification of (c) the dendritic length (n = 56 ± 8 neurons, N = 5 animals); (d) axonal length n = 38 ± 8, N = 3 animals); (e) VAMP2/synaptobrevin-labelled synaptic density (n = 34 ± 7 neurons, N = 3 animals); (f) of VAMP2/synaptobrevin staining intensity n = 34 ± 7 neurons, N = 3 animals). (g) synaptophysin staining intensity (n = 34 ± 7 neurons, N = 3 animals). (f) Bassoon staining intensity (n = 20 ± 1 neurons, N = 2 animals). Detailed information (average, SEM, n and statistics) is shown in Supplementary Table S1.