Table 1.
Overview of the effect of the different shRNAs against VPS35 in the measured presynaptic variables compared to control. Observed changed are bold. Detailed information (average, SEM, n and statistics) is displayed in Supplementary Table S1.
| Figure | measured variable | shVPS35-1 | shVPS35-2 | shVPS35-3 |
|---|---|---|---|---|
| 3C | Dendritic length (µm) | unchanged | unchanged | unchanged |
| 3D | Axonal length (µm) | unchanged | unchanged | unchanged |
| 3F | Synapses/µm (VAMP2) | unchanged | decreased | decreased |
| 3G | VAMP2 (a.u.) | unchanged | unchanged | unchanged |
| 3H | Synaptophysin-1 (a.u) | unchanged | unchanged | unchanged |
| 3I | Synapses/µm (bassoon) | unchanged | unchanged | unchanged |
| 4B | Active zone length (µm) | unchanged | unchanged | unchanged |
| 4C | # Synaptic vesicles/synapse | unchanged | unchanged | unchanged |
| 4D | # docked synaptic vesicles/synapse | unchanged | unchanged | unchanged |
| 5C | 1st peak amplitude (∆F/Fmax) | N.A. | decreased | unchanged |
| 5D | % Active synapses | N.A. | unchanged | unchanged |
| 5E | Fmax (a.u.) | N.A. | decreased | decreased |
| 6C | 1st peak amplitude (∆F/Fmax) | unchanged | unchanged | unchanged |
| 6D | % Active synapses | decreased | unchanged | unchanged |
| 6E | Fmax (a.u.) | decreased | unchanged | unchanged |
| 6F | F Baseline (a.u) | decreased | unchanged | unchanged |
| 6G | Ratio peak amplitude (2nd/1st) | unchanged | unchanged | unchanged |
| 6H | F pH = 5.5 (a.u.) | unchanged | unchanged | unchanged |