Figure 4.
Confluence of A549 and H358 cells over 72 hours after seeding at different initial densities when the volume of culture media was increased to 3 mL. Shown are the changes in log10 confluence over time measured by live content cell imaging Incucyte HD system (Essen Bioscience) when A549 and H358 cells were seeded at a density of 8 × 105, 4 × 105, 3 × 105 and 2 × 105 cells/well. For this single experiment, measurements were performed in triplicate for control and treated conditions. Shown are the mean ± SEM for the 3 technical replicates per cell line and treatment condition. Shaded area denotes the assay window in a prevalent assay where samples would be taken over 48 hours from the time of dosing (24 hours after seeding). Solid lines are a fitted linear model for the exponential growth phase of cells.