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Chromatographic gradient elution profile for amino acid analysis.
| Time (mins) | Flow(mL/min) | A (%) | B (%) | C (%) | D (%) |
|---|---|---|---|---|---|
| 0.00 | 0.70 | 10.00 | 0.00 | 90.00 | 0.00 |
| 0.29 | 0.70 | 9.90 | 0.00 | 90.10 | 0.00 |
| 5.49 | 0.70 | 9.00 | 80.00 | 11.00 | 0.00 |
| 7.10 | 0.70 | 8.00 | 15.60 | 57.90 | 18.50 |
| 7.30 | 0.70 | 8.00 | 15.60 | 57.90 | 18.50 |
| 7.69 | 0.70 | 7.80 | 0.00 | 70.90 | 21.30 |
| 7.99 | 0.70 | 4.00 | 0.00 | 36.30 | 59.70 |
| 8.59 | 0.70 | 4.00 | 0.00 | 36.30 | 59.70 |
| 8.68 | 0.70 | 10.00 | 0.00 | 90.00 | 0.00 |
| 10.20 | 0.70 | 10.00 | 0.00 | 90.00 | 0.00 |
