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. 2018 Feb 14;8:2985. doi: 10.1038/s41598-018-21460-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The siRNA knockdown of RACK1 protected Marc-145 cells from cytopathic effects and reduced the viral titer 40-fold compared with the non-targeting knockdown. Marc-145 cells (10⁴ cells per well) were seeded into 96-well plates the day of transfection. Forty eight hours post transfection, ten-fold serial dilution of PRRSV YN-1 strain was prepared in medium. One hundred µl/well of each dilution was added into 6 wells in total. CPE was recorded using the inverted microscope over a period of 3 days post transfection. The 50% tissue culture infected dose (TCID₅₀) was determined by Reed–Muench method. Compared with the siScramble control group, transfection with siRACK1 significantly protected Marc-145 cells from cytopathic effects (A) and reduced the viral titer by nearly 40 times. These data is representative for three independent experiments.