Table 1.
Oligonucleotides used in this study.
| Name | Sequence 5′—3′) |
|---|---|
| RACK1_F_seq | CGCCACCATGACTGAGCAGAT |
| RACK1_R_seq | CTAGCGCGTGCCGATGGT |
| siRACK1_1 (sense) | ccaucaagcuauggaauacdTdT |
| siRACK1_1 (antisense) | guauuccauagcuugauggdTdT |
| siRACK1_2 (sense) | caaauacacuguccaggaudTdT |
| siRACK1_2 (antisense) | auccuggacaguguauuugdTdT |
| siScramble (sense) | uucuccgaacgugucacgudTdT |
| siScramble (antisense) | acgugacacguucggagaadTdT |
| RACK1_F | TTCTCCTCTGACAACCGGCA |
| RACK1_R | GCCATCCTTGCCTCCAGAA |
| Viral N_F | AATGGCCAGCCAGTCAATCA |
| Viral N_R | TCATGCTGAGGGTGATGCTG |
| IFNα_F | gtgctcagctgcaagtcaag |
| IFNα_R | tggtttgagccttttggaac |
| GAPDH_F | TGGAAAAACCTGCCAAGTACG |
| GAPDH_R | ATGAGGTCCACCACCCTGTT |
CGCCACC, as the conserved sequence of Kozak sequence, is tagged to the 5′ of the start codon of the forward primer sequence to amplify the full sequence of the RACK1 mRNA.