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. 2018 Feb 14;8:3013. doi: 10.1038/s41598-018-21273-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The distribution of inflammatory cells changes in the resolution process. In the IVC-stenosis model thrombus weight decreases during a time period of 21 days (A) as well as the total cell count of CD45⁺ cells/mg thrombus (B). Results are shown as median with interquartile range, n = 5 to 6 animals per group. One way-ANOVA/Bonferroni’s Multiple Comparison test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. The flow-cytometric analysis of CD45⁺CD11b⁺Gr-1⁺ neutrophils, CD45⁺/CD11b⁺/Gr-1⁻ monocytes and CD45⁺/CD11b⁻/TCRβ⁺ T-cells in thrombus tissue reveals different peaks of these cells during a time course of day 2, 6, 10 and 21 (C). Results are shown as mean ± SEM, n = 5 to 6 animals per group. One way-ANOVA/Bonferroni’s Multiple Comparison test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. (D) Representative FACS-plots of CD11b⁺/Gr-1⁺ neutrophils (blue), CD11b⁺/Gr-1⁻ monocytes (yellow) and NK1.1⁻/TCR beta⁺ T-cells in thrombus material (day 2, 6, 10, 21 post subtotal ligation) of C57BL/6 mice.