Figure 1.

In vitro FCFM of different fungal species. The images were acquired by making direct contact between the fibre-optic probe (S-1500) and the fungal colonies grown on solid agar. (A–C) Representative FCFM images of a WT A. fumigatus colony, a WT C. gattii colony and a non-infected region on the agar showing the absence of background signals. (D–F) Representative FCFM images of a GFP-expressing A. fumigatus colony, a GFP-expressing C. gattii colony and a non-infected region on the agar showing the presence of fungal hyphae (Aspergillus), cells (C. gattii) or the lack of background signals, respectively.