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. 2018 Feb 14;8:3009. doi: 10.1038/s41598-018-20545-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In situ, transthoracic FCFM of lung infections. The images were acquired by placing the fibre-optic probe (S-1500) into direct contact with the lung surface after performing a bilateral thoracotomy. (A–C) Representative FCFM images of a healthy appearing area of an A. fumigatus (GFP-expressing) infected lung, a C. gattii (GFP-expressing) infected lung and a non-infected control lung. (D,E) Representative FCFM images of an infected area of an A. fumigatus infected lung and a C. gattii infected lung, showing the presence of fungal cells.