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. 2018 Feb 14;8:3009. doi: 10.1038/s41598-018-20545-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative analysis of the size distribution of cells detected on the in vivo bronchoscopic FCFM images. The average cell size per frame of all frames and animals were pooled per time point to obtain a relative frequency distribution of cell sizes. Frames containing zero detected cells or having a mean signal intensity lower than 5 were excluded from the analysis. Histograms (bin width of 10 µm²) representing the average cell size per frame on day 13, 17, 21 and 24 post instillation with C. gattii (top row) or C. neoformans (bottom row) show a wider variation in cell sizes for C. neoformans.